Oxidative desulfurization pathway for complete catabolism of sulfoquinovose by bacteria

M Sharma; JP Lingford; M Petricevic; AJD Snow; Y Zhang; MA Järvå; JWY Mui; NE Scott; EC Saunders; R Mao; R Epa; BM da Silva; DEV Pires; DB Ascher; MJ McConville; GJ Davies; SJ Williams; ED Goddard-Borger

Journal article

Oxidative desulfurization pathway for complete catabolism of sulfoquinovose by bacteria

M Sharma, JP Lingford, M Petricevic, AJD Snow, Y Zhang, MA Järvå, JWY Mui, NE Scott, EC Saunders, R Mao, R Epa, BM da Silva, DEV Pires, DB Ascher, MJ McConville, GJ Davies, SJ Williams, ED Goddard-Borger

Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD SCIENCES | Published : 2022

DOI: 10.1073/pnas.2116022119

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Abstract

Catabolism of sulfoquinovose (SQ; 6-deoxy-6-sulfoglucose), the ubiquitous sulfosugar produced by photosynthetic organisms, is an important component of the biogeochemical carbon and sulfur cycles. Here, we describe a pathway for SQ degradation that involves oxidative desulfurization to release sulfite and enable utilization of the entire carbon skeleton of the sugar to support the growth of the plant pathogen Agrobacterium tumefaciens. SQ or its glycoside sulfoquinovosyl glycerol are imported into the cell by an ATP-binding cassette transporter system with an associated SQ binding protein. A sulfoquinovosidase hydrolyzes the SQ glycoside and the liberated SQ is acted on by a flavin mononucle..

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University of Melbourne Researchers

Nichollas Scott Author

Eleanor Saunders Author

Ruwan Epa Author

Douglas Valente Pires Author

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Grants

Awarded by University of Melbourne

Funding Acknowledgements

Dr. Monica Doblin is thanked for the provision of A. tumefaciens strain C58. This work was supported in part by National Health and Medical Research Council of Australia (NHMRC) project grants GNT1100164 (N.E.S) , GNT1174405 (D.B.A.) , and GNT1139546 and GNT1139549 (E.D.G.-B.) ; the Leverhulme Trust grant RPG-2017-190 (G.J.D.) ; Australian Research Council grants DP180101957 and DP210100233 (S.J.W.) and DP210100362 (N.E.S.) ; and support from The Walter and Eliza Hall Institute of Medical Research, the Australian Cancer Research Fund, and a Victorian State Government Operational Infrastructure support grant (E.D.G.-B.) . G.J.D. is sup-ported by the Royal Society Ken Murray Research Professorship, E.D.G.-B. is supported by the Brian M. Davis Charitable Foundation Centenary Fellowship, M.J.M. is an NHMRC Principal Research Fellow, N.E.S. is supported by an Australian Research Council Future Fellowship (FT200100270) , B.M.d.S. was supported by the Melbourne Research Scholarship, J.W.Y.M. was supported by a Sir John and Lady Higgins Scholarship, and M.P. was supported by an Aus-tralian Postgraduate Award. We acknowledge Dr. Johan P. Turkenburg and Sam Hart for assistance with X-ray data collection; the Diamond Light Source for access to beamlines I04, I24, and I04-1 under proposal number mx-18598; and the Australian Synchrotron, part of Australia's Nuclear Science and Tech-nology Organisation, for access to the MX-2 beamline, which made use of the Australian Cancer Research Foundation detector. We thank the Melbourne Mass Spectrometry and Proteomics Facility of the Bio21 Molecular Science and Biotechnology Institute at The University of Melbourne for the support of mass spectrometry analysis and the Bioscience Technology Facility (University of York) for assistance with SEC-MALS analyses.